The endometrial cancer detection using non-invasive hypermethylation of CDO1 and CELF4 genes in women with postmenopausal bleeding in Northwest China
TL;DR 摘要总结
研究通过检测宫颈脱落细胞中CDO1和CELF4基因的甲基化水平，评估其在绝经后出血女性中诊断子宫内膜癌的临床价值。结果显示该方法灵敏度为87.5%，特异度为95.9%，对II型子宫内膜癌检出率达100%。该非侵入性检测方法具有高特异度，可作为辅助诊断工具帮助医生区分绝经后出血患者的良恶性病变。
摘要 · Abstract
OBJECTIVE: The objective of this study was to verify the clinical predictive performance of methylated cysteine dioxygenase type 1 (CDO1m) and CUGBP Elav-like family member 4 (CELF4m) in endometrial cancer (EC) women with postmenopausal bleeding (PMB). MATERIAL AND METHODS: A single-center, prospective, and case-control study was conducted in the Gansu Provincial Maternity and Child-care Hospital with 138 female postmenopausal patients enrolled in 2022. All patients underwent body mass index (BMI) detection, transvaginal ultrasonography (TVUS) detection, carbohydrate antigen 125 detection, and the cervical exfoliated cell CDO1/CELF4 gene methylation detection to analyze the sensitivity, specificity, and accuracy of different screening tests statistically with the biopsy and/or dilation and curettage (D&C) pathological diagnosis under hysteroscopy as the gold standard
RESULTS: There was no significant difference in age between the EC group and the non-EC group, P = 0.492. Using quantitative polymerase chain reaction (qPCR) technology, we validated the CDO1 and CELF4 methylation detection with 87.5% sensitivity and 95.9% specificity as a useful strategy for the triage of women with PMB for the detection of EC. In addition, 100% of type II EC (n = 6) were positively detected by the CDO1 or CELF4 methylation test
CONCLUSION: The CDO1 and CELF4 methylation test with high specificity as an auxiliary diagnostic tool or alternative method provides physicians with a reference to distinguish between benign and malignant tumors in women with postmenopausal bleeding, to justify the necessity of using invasive methods to confirm diagnosis.
发表单位
Gansu Provincial Maternity and Child-care Hospital
引用格式
Cai, B. et al. The endometrial cancer detection using non-invasive hypermethylation of CDO1 and CELF4 genes in women with postmenopausal bleeding in Northwest China. Cytojournal 21, 15 (2024).
文献海报 · Poster
在新窗口查看
* 海报为中英双语，标题双语对照显示；可在海报右上角切换 EN / 中文，语言自动适配当前页面。