Triage performance of DNA methylation for women with high-risk human papillomavirus infection
TL;DR 摘要总结
DNA甲基化标志物PAX1m/JAM3m在宫颈癌筛查中表现优异。研究对比了该标志物与HPV16/18基因分型和液基细胞学检测在1105名高危HPV感染女性中检测宫颈上皮内瘤变3级及以上病变的性能。结果显示PAX1m/JAM3m特异性最高（89.6%），诊断效能最强（AUC=0.790），可显著减少阴性患者的阴道镜转诊，是优于现有方法的分流生物标志物。
摘要 · Abstract
Objective DNA methylation is a promising biomarker for cervical cancer screening. This study aimed to validate the triage performance of cytological DNA methylation for detecting cervical intraepithelial neoplasia of grade 3 or worse (CIN3+) in women with high-risk human papillomavirus (hrHPV) infection from a large prospective cohort undergoing opportunistic screening in China (METHY3)
Methods The triage performance for detecting CIN3+ lesions was compared between HPV16/18 genotyping, a liquid-based cytology (LBC) test, and the PAX1 and JAM3 methylation (PAX1m/JAM3m) test according to cervical pathologic outcomes. Among the 4394 women infected with hrHPV, 1105 had definitive cervical histological findings that were analyzed
Results For detecting CIN3+, the specificity of HPV16/18(+), the LBC result of ��atypical squamous cells of undetermined significance (ASCUS), and PAX1m/JAM3m(+) was 66.4%, 23.9%, and 89.6%, respectively, with odds ratios of 4.24 (95% confidence interval [CI], 2.85-6.40), 4.44 (2.27-10.1), and 18.5 (12.1-28.7) (P?<?.001), respectively. PAX1m/JAM3m(+) had the highest area under the receiver operating characteristic curve (0.790, 95% CI, 0.747-0.832) in the whole cohort and in women of various ages. PAX1m/JAM3m (+) was detected in all patients with cancer (n?=?28). Compared with HPV16/18 genotyping and the LBC test, PAX1m/JAM3m testing reduced referrals to colposcopy by 20.64 percentage points and 61.18 percentage points, respectively
Conclusions PAX1m/JAM3m testing is highly specific for detecting CIN3+. As a triage biomarker, it is superior to HPV 16/18 genotyping and LBC testing for women with hrHPV infection.
发表单位
Peking Union Medical College Hospital
引用格式
Kong, L. et al. Triage performance of DNA methylation for women with high-risk human papillomavirus infection. The Oncologist 30, oyae324 (2025).
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