Preliminary study on PAX1/JAM3 methylation and HPV viral load in CIN3-like squamous cell carcinoma: Are there differences from CIN3 and early invasive carcinoma?
TL;DR 摘要总结
研究对比分析了宫颈CIN3、CIN3样鳞癌、早期浸润癌和浸润癌中PAX1/JAM3甲基化水平和HPV病毒载量的差异。结果显示，PAX1和JAM3甲基化水平在癌症中显著高于高级别病变，但CIN3样鳞癌与早期浸润癌间无明显差异；CIN3样鳞癌的HPV病毒载量显著高于CIN3和早期浸润癌，提示其具有独特的分子生物学特征。
摘要 · Abstract
Objective Cervical intraepithelial neoplasia grade 3 (CIN 3)-like squamous cell carcinoma (SCC) is a recently identified subtype of cervical cancer with a deceptive growth pattern. It mimics the phenotype of CIN 3, involving endocervical crypts, presenting significant challenges and potential for diagnostic confusion. This study aims to clarify the differences in epigenetic profiles and HPV viral load(VL) among CIN 3, CIN 3-like SCC, early invasive SCC, and invasive SCC
Methods A retrospective analysis was conducted on 138 cases of cervical high-grade lesions and SCC diagnosed at Peking University People's Hospital from March 2022 to June 2024. The cases included 65 high-grade squamous intraepithelial lesions (HSIL) [23 CIN 2 and 42 CIN 3] and 73 SCC [27 CIN 3-like SCC, 21 early invasive SCC, and 25 invasive SCC]. Histological samples were analyzed for methylation levels of the epigenetic factors PAX1 and JAM3, as well as HPV VL
Results Methylation levels of PAX1 and JAM3, as well as total HPV VL, were significantly higher in cervical cancer than in HSIL(<?0.001). The PAX1 methylation level of CIN3 was significantly lower than that of invasive SCC, but there was no significant difference compared with CIN3 and CIN 3-like SCC; For the PAX1/JAM3 methylation levels in CIN 3-like SCC, no statistically significant differences were observed when compared with those in early invasive SCC and invasive SCC. The HPV VL in CIN 3-like SCC was significantly higher than that in CIN 3 and early invasive SCC, but no significant difference was found compared with invasive SCC. In the same stage of FIGO, although PAX1/JAM3 methylation and HPV VL (especially HPV16/18 VL) showed a tendency to increase in CIN3-like SCC compared with early invasive SCC, the difference was not significant, further expansion of sample size is needed to confirm this
Conclusion PAX1 and JAM3 methylation levels are significantly higher in SCC than HSIL, but no difference in methylation levels was observed between CIN 3-like SCC, early invasive SCC, and invasive SCC. Total HPV VL (especially HPV16/18 VL) in CIN3-like SCC is significantly higher than that in CIN3 and early invasive SCC, suggesting potential shared and distinct features in epigenetics and HPV VL between CIN3-like SCC and early invasive SCC, which need more molecular biology research to explore the difference.
发表单位
北京大学人民医院
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